2013) and bioconversion of lignocelluloses into feedstocks and fuels ( Kim et al 2000). Xylanolytic enzymes are receiving increasing attention because of their potential application in pulp bleaching (Golugiri et al. Xylanases and associated debranching enzymes produced by a variety of microorganisms including bacteria, yeast and filamentous fungi, bring about the hydrolysis of hemicelluloses ( Maheshwari et al. This complex heteropolysaccharide consists of a main chain of 1,4-β-D-xylose monomers and short chain branches consisting of O-acetyl, α-L-arabinofuranosyl and α-D-glucuronyl residues. Xylan is the major hemicellulosic constituent of hard and soft wood, and is the next most abundant renewable polysaccharide after cellulose.
#Table format in clc sequence viewer free
Key words: Cellulase free xylanase Cellulosimicrobium cellulans E.coli Cloning Gene Secondary and homology based 3D structure of xylanase was analysed using SOPMA and Swiss-Prot software. In silico physico-chemical characterization of amino acid sequence of xylanase showed an open reading frame encoding a 556 amino acid sequence with a molecular weight of 58 kDa and theoretical isolectric point (pI) of 4.46 was computed using Expasy's ProtParam server. FJ859907.1) reported from the gut of Eisenia fetida in Korea. It showed 98% homology withCellulosimicrobium sp. A BLAST search confirmed that the DNA sequence from the amplified fragment was endo-1, 4-beta-xylanase, which was a member of glycoside hydrolase family 11. A distinct fragment of about 1671 bp was successfully amplified using PCR and cloned into Escherichia coli DH5α. Due to the xylanolytic activity of this bacterium, isolation and characterization of the xylanase gene were attempted. Results from the metabolic fingerprinting, whole-cell fatty acids methyl ester analysis and 16S rDNA sequencing suggested the bacterium to be Cellulosimicrobium cellulans CKMX1. A xylanolytic bacterium was isolated from mushroom compost by using enrichment technique.
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